Contribution of the CASA system, based on open source software, to the assessment of sperm characteristics: informetric and usage-based approaches
نویسندگان
چکیده
By using informetric tools, we evaluated the contribution and impact of the open source computer-assisted sperm analysis (CASA) software on sperm studies, especially pertaining to fish. The article was cited 125 times between 2007 and 2014 in four publication types (articles, doctoral dissertations, master’s theses, and books). Among articles, the number of citations increased at rate of three citations per year and the calculated h-index was 17. Of the studies that cited Wilson-Leedy and Ingermann (2007), fish were the main group of organisms studied (61 articles); of these, 34 articles used the open source CASA, covering 23 marine or freshwater species. Studies conducted in 12 countries were published in 21 journals. Four ways of knowledge sharing were identified: by direct partnership with the system developers; by knowledge multipliers, who worked directly with the system developers; by groups that adopted the system by their own initiative; and through expansion of cooperation networks with autodidacts. In regard to specific analytical procedures, motility, curvilinear velocity, average path velocity, and straight-line velocity were the main CASA parameters used to describe sperm movements. As expected, the usage of this tool has been increasing over the years, being a reliable and versatile alternative to more costly similar platforms. However, the potential of the CASA system has not been completely explored and it is our assessment that some results produced by CASA are poorly understood or even misunderstood.
منابع مشابه
“Short Paper” Application of Gamma Irradiation to Increase the Motility and Viability of Bovine Sperm after Freezing and Thawing
This study was conducted to determine the suitable dose of gamma rays for increasing sperm motility and viability after thawing and to assess its effects on malondialdehyde concentration and the rate of sperm DNA strand breaks. Sperm samples in liquid nitrogen were irradiated at doses of zero, 0.1, 0.3, 0.5, 0.7 and 0.9 Gy. The sperm quality and quantity parameters were evaluated before and aft...
متن کاملP-95: Flow Cytometry Analysis of Bovine Semen:A Qualitative Study
Background: Although AI practices have been introduced little over 60 years, the success rate remains relatively low. This might be due to the exclusive selection of semen based on motility analysis. Recent advancement in sperm sexing using flow cytometry with an increased throughput from next generation cell sorters, made use of this technology in studding sperm qualitative aspects other than ...
متن کاملComparison of three different staining methods for the morphometric characterization of Alpaca (Vicugna pacos) sperm, using ISAS® CASA-Morph system
Sperm morphometry is a part of the semen analysis based on CASA technology and has shown a big role in the prediction of male fertility. This analysis implies the use of stain techniques, although it has been shown that staining can make dramatic changes in the cell morphometry in different species. The aim of the present work was to evaluate the differences in sperm morphometry in Alpaca, intr...
متن کاملO-14: New Era in Sperm Selection for ICSIProcedure
Background: Intra-cytoplasmic sperm insemination, the ICSI, is considered as the one most routine treatment of male infertility. Although the genetic inheritance of an individual is based on the genetic contribution from both the oocyte and sperm, considering the fact that ICSI by passes all the barriers to natural fertilization much emphasis was given to oocyte quality to improve the ICSI outc...
متن کاملComparison of Open Source Learning Management Softwares and Presenting a Native Evaluation Tool
Introduction: Nowadays all educational institutes are trying to use technology in their structure. This effort has been faced with different barriers, including cost, time, and support. Therefore, using open source softwares can partially help us in using technology. In this article, we review main features of several open source learning management softwares, while presenting a tool which incl...
متن کامل